F1-hybrid anti-parental-strain reactivity. II. Proliferation of C3H X CBA hybrid lymphocytes in the spleens of irradiated CBA mice.

Abstract

Experiments were conducted to explore whether C3H X CBA lymphocytes can react by proliferation when they meet parental CBA spleen cells. MLC tests could not detect such a reaction, but infusion of C3H X CBA lymph node cells into irradiated CBA hosts resulted in rapid cell proliferation in the host's spleen. Such a cell proliferation was also observed after infusion of T-cell-enriched lymph node cell preparations or thymic cells from C3H X CBA donors. Upon transfer to new irradiated mice these proliferating cells continued to proliferate in CBA mice but not in C3H X CBA mice. Further evidence that the injected T-cells were the proliferating emerged from experiments where AKR X CBA lymphocytes were found to proliferate in spleens of irradiated CBA mice and that most of these cells posessed the theta antigen determined by AKR. Proliferation of F1-hybrid lymphocytes in the spleens of its irradiated parental strains was found not to be a general phenomenon and is probably restricted to some Mls-antigen-compatible strain combinations. The possibility that the C3H X CBA hybrid lymphocytes are stimulated to proliferations by CBA lymphocytes reactive against the C3H-determined Mls antigen is discussed.