Critical functions for c-Myb at three checkpoints during thymocyte development.

Timothy P Bender; Christopher S Kremer; Manfred Kraus; Thorsten Buch; Klaus Rajewsky

doi:10.1038/ni1085

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Critical functions for c-Myb at three checkpoints during thymocyte development.

Timothy P Bender·Christopher S Kremer·Manfred Kraus·Thorsten Buch·Klaus Rajewsky

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Abstract

The transcription factor c-Myb is expressed throughout T cell development in the thymus. However, little is understood about c-Myb function because of the embryonic lethality of traditional Myb-null mutations. Using tissue-specific deletion to abrogate c-Myb expression at distinct stages of T cell development, we identify three points at which c-Myb activity is required for normal T cell differentiation: transition through the double-negative 3 stage, survival of preselection CD4(+)CD8(+) thymocytes, and differentiation of CD4 thymocytes. Thus, c-Myb is essential at several stages during T cell development in the thymus.

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Animals CD4-Positive T-Lymphocytes CD8-Positive T-Lymphocytes Cell Differentiation Cell Survival Gene Deletion Gene Expression Regulation Gene Rearrangement, T-Lymphocyte Mice Mice, Transgenic Proto-Oncogene Proteins c-myb RNA, Messenger Recombination, Genetic Thymus Gland Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

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Timothy P Bender, Christopher S Kremer, Manfred Kraus, Thorsten Buch, Klaus Rajewsky. 2004-06-13. Critical functions for c-Myb at three checkpoints during thymocyte development.. https://doi.org/10.1038/ni1085

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