Ruling out Bacillus anthracis.

Joseph Papaparaskevas; Dimitra P Houhoula; Maria Papadimitriou; Georgios Saroglou; Nicholas J Legakis; Loukia Zerva

doi:10.3201/eid1004.030544

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PubMed · 15200872

Ruling out Bacillus anthracis.

Joseph Papaparaskevas·Dimitra P Houhoula·Maria Papadimitriou·Georgios Saroglou·Nicholas J Legakis·Loukia Zerva

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Abstract

Optimization of methods for ruling out Bacillus anthracis leads to increased yields, faster turnaround times, and a lighter workload. We used 72 environmental non-B. anthracis bacilli to validate methods for ruling out B. anthracis. Most effective were the use of horse blood agar, motility testing after isolates had a 2-h incubation in trypticase soy broth, and screening isolates with a B. anthracis-selective agar.

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Animals Bacillus anthracis Bioterrorism Culture Media Hemolysis Humans Polymerase Chain Reaction Retrospective Studies Journal Article Research Support, Non-U.S. Gov't

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Joseph Papaparaskevas, Dimitra P Houhoula, Maria Papadimitriou, Georgios Saroglou, Nicholas J Legakis, Loukia Zerva. 2004. Ruling out Bacillus anthracis.. https://doi.org/10.3201/eid1004.030544

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