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PubMed · 15205561

Decrease of interleukin-10-producing T cells in the peripheral blood of severe unstable atopic asthmatics.

Koichiro Matsumoto·Hiromasa Inoue·Satoru Fukuyama·Miyuki Tsuda·Tomomi Ikegami·Atsuko Kibe·Yuki Yoshiura·Masashi Komori·Naotaka Hamasaki·Hisamichi Aizawa·Yoichi Nakanishi
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Abstract

BACKGROUND: Although IL-10 is known as an immunoregulatory cytokine produced by various cells including T cells, its basic profile in atopic asthma remains uncertain. OBJECTIVE: The profiles of IL-10 production in circulating CD4+ T cells of atopic asthmatics were investigated with respect to clinical severity. METHODS: Forty atopic asthmatics were divided into three groups: mild, and severe but stable and severe unstable asthmatics. Eosinophils were counted in the peripheral blood and sputum, and exhaled nitric oxide was assessed. PBMCs were stimulated with or without anti-CD3 and anti-CD28 antibodies and then processed for detecting IL-10-producing CD4+ cells using flow cytometry. RESULTS: There was no difference in the eosinophil count in blood or sputum and in nitric oxide level among the three groups. IL-10-producing CD4+ cells were mainly detected in a CD45RO+ memory population. The frequency of IL-10-producing cells after stimulation was significantly lower in the severe unstable group compared to the mild group. In addition, the frequency of IL-10-producing cells in the severe unstable group was significantly lower than that in the severe stable group despite the fact that both groups received similar treatments with high-dose inhaled corticosteroids. The IL-10 production of CD4+CD45RO+ cells in response to dexamethasone did not differ among the three groups. CONCLUSIONS: IL-10-producing CD4+CD45RO+ cells in the peripheral blood are decreased in severe unstable asthmatics, which is not explained by the effect of high-dose inhaled corticosteroid medication.

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AdultAsthmaBiomarkersBronchial Provocation TestsCD4-Positive T-LymphocytesDexamethasoneFemaleFlow CytometryForced Expiratory VolumeGlucocorticoidsHumansHypersensitivity, ImmediateInterleukin-10Leukocyte Common AntigensLeukocytes, MononuclearMaleMiddle AgedSeverity of Illness IndexSputumT-Lymphocyte SubsetsComparative StudyJournal ArticleResearch Support, Non-U.S. Gov't

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Koichiro Matsumoto, Hiromasa Inoue, Satoru Fukuyama, Miyuki Tsuda, Tomomi Ikegami, Atsuko Kibe, Yuki Yoshiura, Masashi Komori, Naotaka Hamasaki, Hisamichi Aizawa, Yoichi Nakanishi. 2004-06-17. Decrease of interleukin-10-producing T cells in the peripheral blood of severe unstable atopic asthmatics.. https://doi.org/10.1159/000079167

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