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PubMed · 1520633

A rapid solid-phase extraction and HPLC/DAD procedure for the simultaneous determination and quantification of different benzodiazepines in serum, blood and post-mortem blood.

F Musshoff·T Daldrup
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Abstract

A rapid and quantitative method for the determination of benzodiazepines using high-performance liquid chromatography (HPLC) with diode-array detection (DAD) is reported. The drugs were extracted from serum, blood or post-mortem blood using C18 extraction columns. Brotizolam was used as internal standard. Experiments with spiked serum/blood samples resulted in recoveries between 75% and 94% for all investigated benzodiazepines. Excellent linearity was obtained over the concentration range 5-1500 ng benzodiazepine/ml. The limit of detection was approximately 2 ng/ml. The detection of low therapeutic serum levels of highly potent benzodiazepines is also possible.

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BenzodiazepinesChromatography, High Pressure LiquidEvaluation Studies as TopicForensic MedicineHumansPostmortem ChangesReproducibility of ResultsSensitivity and SpecificitySubstance Abuse DetectionJournal Article

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F Musshoff, T Daldrup. 1992. A rapid solid-phase extraction and HPLC/DAD procedure for the simultaneous determination and quantification of different benzodiazepines in serum, blood and post-mortem blood.. https://doi.org/10.1007/bf02340833

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PubMed2006

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