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PubMed · 15209342

Innovative instrumentation for microarray scanning and analysis: application for characterization of oligonucleotide duplexes behavior.

E B Khomyakova·E V Dreval·M Tran-Dang·M C Potier·F P Soussaline
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Abstract

Accuracy in microarray technology requires new approaches to microarray reader development. A microarray reader system (optical scanning array or OSA reader) based on automated microscopy with large field of view, high speed 3 axis scanning at multiple narrow-band spectra of excitation light has been developed. It allows fast capture of high-resolution, multi-fluorescence images and is characterized by a linear dynamic range and sensitivity comparable to commonly used photo-multiplier tube (PMT)-based laser scanner. Controlled by high performance software, the instrument can be used for scanning and quantitative analysis of any type of dry microarray. Studies implying temperature-controlled hybridization chamber containing a microarray can also be performed. This enables the registration of kinetics and melting curves. This feature is required in a wide range of on-chip chemical and enzymatic reactions including on-chip PCR amplification. We used the OSA reader for the characterization of hybridization and melting behaviour of oligonucleotide:oligonucleotide duplexes on three-dimensional Code Link slides.

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Image Processing, Computer-AssistedKineticsMicroscopy, FluorescenceNucleic Acid HybridizationOligonucleotide Array Sequence AnalysisOligonucleotidesSoftwareEvaluation StudyJournal ArticleResearch Support, Non-U.S. Gov't

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E B Khomyakova, E V Dreval, M Tran-Dang, M C Potier, F P Soussaline. 2004. Innovative instrumentation for microarray scanning and analysis: application for characterization of oligonucleotide duplexes behavior.. https://pubmed.ncbi.nlm.nih.gov/15209342/

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