NMR difference spectroscopy with a dual saddle-coil difference probe.

Megan A Macnaughtan; Aaron P Smith; Peter B Goldsbrough; Robert E Santini; Daniel Raftery

doi:10.1007/s00216-003-2374-3

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NMR difference spectroscopy with a dual saddle-coil difference probe.

Megan A Macnaughtan·Aaron P Smith·Peter B Goldsbrough·Robert E Santini·Daniel Raftery

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Abstract

A new difference probe for nuclear magnetic resonance (NMR) spectroscopy is presented. The difference probe uses two saddle-shaped coils to excite and detect two samples simultaneously. The samples are held in a specially modified 3-mm NMR tube with an Ultem plastic disk to separate the samples. The probe's resonant circuit contains two crossed diodes that passively switch the relative phase of each coil during the NMR experiment. The result is a difference spectrum from the two samples. The degree of cancellation of common signals was determined to be approximately 90%, and the application of the probe to relaxation-edited difference spectroscopy for identifying protein-ligand interactions was demonstrated using glutathione and glutathione S-transferase binding protein.

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Glutathione Glutathione Transferase Ligands Nuclear Magnetic Resonance, Biomolecular Protein Binding Serine Signal Processing, Computer-Assisted Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

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Megan A Macnaughtan, Aaron P Smith, Peter B Goldsbrough, Robert E Santini, Daniel Raftery. 2004. NMR difference spectroscopy with a dual saddle-coil difference probe.. https://doi.org/10.1007/s00216-003-2374-3

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