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Functionalised electrode array for the detection of nitric oxide released by endothelial cells using different NO-sensing chemistries.

Joshua Oni·Alain Pailleret·Sonnur Isik·Nizam Diab·Ina Radtke·Andrea Blöchl·Michael Jackson·Fethi Bedioui·Wolfgang Schuhmann
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Abstract

In a preliminary study aimed at developing strategies for the simultaneous detection of various biologically important molecules, a procedure is described that allows the electrochemical detection of nitric oxide (NO) released by a population of human umbilical vein endothelial cells (HUVEC) by using an array of electrodes comprising three individually addressable electrodes. Each electrode in the array was modified with a different NO-sensitive electrocatalyst, thereby demonstrating the possibility of modifying the individual electrodes in an array with different sensing chemistries. This study opens a doorway to the development of arrays of electrodes for the simultaneous detection of multiple analytes in a complex environment by suitably tailoring the sensitivity and selectivity of each electrode in the array to a specific analyte in the test medium.

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Biosensing TechniquesCells, CulturedElectrochemistryElectrodesEndothelial CellsHumansNitric OxideOxidation-ReductionPlatinumSurface PropertiesJournal ArticleResearch Support, Non-U.S. Gov't

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BibTeXRIS

Joshua Oni, Alain Pailleret, Sonnur Isik, Nizam Diab, Ina Radtke, Andrea Blöchl, Michael Jackson, Fethi Bedioui, Wolfgang Schuhmann. 2004. Functionalised electrode array for the detection of nitric oxide released by endothelial cells using different NO-sensing chemistries.. https://doi.org/10.1007/s00216-004-2512-6

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