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PubMed · 15217763

[Plasma ascorbic acid: preanalytical factors and new HPLC method].

F Flourié·J-P Steghens
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Abstract

We describe a fast ED-HPLC method for ascorbic acid analysis, carried out on a new mixed mode chromatographic column. Due to its functional dodecylsilane group associating a quaternary ammonium, this column is simpler and easier to handle than previous ones because without any counter-ion in the mobile phase. As ascorbic acid and glutathione are very often measured on the same blood sample, it was efficient to validate a common pretreatment solution for ascorbic acid in plasma and glutathione in whole blood. This pretreatment is based on the use of a sulfosalycilic acid, N-ethylmaleimide, EDTA mix recently described. Plasma samples, stored after deproteinisation and without centrifugation, are stable for three weeks at -20 or -80 degrees C.

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Ascorbic AcidChromatography, High Pressure LiquidComparative StudyEnglish AbstractJournal Article

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F Flourié, J-P Steghens. [Plasma ascorbic acid: preanalytical factors and new HPLC method].. https://pubmed.ncbi.nlm.nih.gov/15217763/

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Effect of light on ascorbic acid biosynthesis and bioinformatics analysis of related genes in Chinese chives.

Ascorbic acid (AsA) is an essential nutritional component and powerful antioxidant in vegetables, and in plants, AsA levels are regulated by light. AsA levels in the leaves of Chinese chive (Allium tuberosum Rottler ex Spr), a popular vegetable, are poorly understood. Thus, this study was performed to assess the influence of light on AsA biosynthesis in chive and select related genes (AtuGGP1 and AtuGME1); in addition, bioinformatic analyses and gene expression level assays were performed. The biological information obtained for AtuGGP1 and AtuGME1 was analysed with several tools, including NCBI, DNAMAN, and MEGA11. After different light treatments were performed, the Chive AsA content and AtuGGP1 and AtuGME1 expression levels were determined. These results suggest that 1) compared with natural light, continuous darkness inhibited AsA synthesis in chives. 2) The amino acid sequences of AtuGGP1 and AtuGME1 are very similar to those of other plants. 3) The trends observed for the expression levels of AtuGGP1 and AtuGME1 were consistent with the AsA content observed in chives. Hence, we speculated that light controls AsA biosynthesis in chives by regulating AtuGGP1 and AtuGME1 expression. This study provided impactful and informative evidence regarding the functions of GGP and GME in chives.

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