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Plexin-B3 is a functional receptor for semaphorin 5A.

Stefania Artigiani·Paolo Conrotto·Pietro Fazzari·Giorgio F Gilestro·Davide Barberis·Silvia Giordano·Paolo M Comoglio·Luca Tamagnone
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Abstract

Semaphorins are a large family of molecular cues implicated in neural development and in a variety of functions outside the nervous system. Semaphorin 5A (Sema5A) is a transmembrane semaphorin, containing seven thrombospondin type-1 repeats, which was recently found to control axon guidance. Here we show that plexin-B3 is a high-affinity receptor specific for Sema5A. We further demonstrate that plexin-B3 activation by Sema5A mediates functional responses in plexin-B3-expressing cells (either fibroblasts, epithelial and primary endothelial cells). In addition, Sema5A can trigger the intracellular signalling of the hepatocyte growth factor/scatter factor receptor, Met, associated in a complex with plexin-B3. We thus conclude that Sema5A is able to elicit multiple functional responses through its receptor plexin-B3.

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AnimalsAxonsBlotting, WesternCOS CellsCell LineCell MembraneCell MovementDose-Response Relationship, DrugEndothelium, VascularEpithelial CellsFibroblastsHumansImmunoprecipitationKineticsMembrane ProteinsMiceNIH 3T3 CellsNerve Tissue ProteinsNeural Cell Adhesion MoleculesProtein BindingProto-Oncogene Proteins c-metRNA, Small InterferingReceptors, Cell SurfaceSemaphorinsSignal TransductionJournal ArticleResearch Support, Non-U.S. Gov't

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Stefania Artigiani, Paolo Conrotto, Pietro Fazzari, Giorgio F Gilestro, Davide Barberis, Silvia Giordano, Paolo M Comoglio, Luca Tamagnone. 2004-06-25. Plexin-B3 is a functional receptor for semaphorin 5A.. https://doi.org/10.1038/sj.embor.7400189

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