Assaying Cdc25 phosphatase activity.

Ingo Hassepass; Ingrid Hoffmann

doi:10.1385/1-59259-811-0:153

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PubMed · 15220527

Assaying Cdc25 phosphatase activity.

Ingo Hassepass·Ingrid Hoffmann

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Abstract

To determine the activity of all human Cdc25 phosphatases, two different methods are described. For assaying phosphatase activities of recombinant Cdc25 proteins produced in Escherichia coli or insect cells, a fluorimetric assay using fluorescein diphosphate (FDP) as a substrate is recommended. To analyze endogenous Cdc25 phosphatase activities of immunoprecipitates from total cellular extracts, the physiological substrate Cdk1/cyclin B1 is most sensitive.

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Animals CDC2 Protein Kinase Cyclin B Cyclin B1 Escherichia coli Fluorescein Glutathione Transferase HeLa Cells Humans Phosphorylation Protein Kinases Recombinant Fusion Proteins S Phase Spodoptera cdc25 Phosphatases Journal Article Research Support, U.S. Gov't, P.H.S.

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Ingo Hassepass, Ingrid Hoffmann. 2004. Assaying Cdc25 phosphatase activity.. https://doi.org/10.1385/1-59259-811-0%3A153

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