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PubMed · 15221195

Specific polyclonal-based immunoassays for sulfathiazole.

Nuria Pastor-Navarro·Cristina García-Bover·Angel Maquieira·Rosa Puchades
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Abstract

A highly sensitive and specific enzyme-linked immunosorbent assay has been developed for detection of sulfathiazole (STZ, 4-amino- N-thiazol-2-yl-benzenesulfonamide). A set of haptens was synthesized in order to produce polyclonal antibodies against sulfonamides. Two ELISA formats (antibody-coated and conjugate-coated) were also investigated using all the serum/coating conjugate combinations that showed specific recognition. The developed ELISA succeeded in detection of STZ at concentrations as low as 0.03 ng mL(-1) over a measurable range of 0.12-6.71 ng mL(-1). Selectivity studies have demonstrated that other sulfonamides do not interfere significantly (<10%) with analysis of STZ by this immunochemical technique. Analysis of spiked bee honey samples by the developed ELISA method showed recoveries were good. The selectivity and sensitivity (IC(50)=1.6 ng mL(-1)) make it a suitable screening method for determination of low levels of STZ in food samples.

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AntibodiesAntibody SpecificityEnzyme-Linked Immunosorbent AssayHaptensHoneyHydrogen-Ion ConcentrationImmunoassayMolecular StructureSensitivity and SpecificitySulfathiazoleSulfathiazolesJournal ArticleResearch Support, Non-U.S. Gov't

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Nuria Pastor-Navarro, Cristina García-Bover, Angel Maquieira, Rosa Puchades. 2004-06-18. Specific polyclonal-based immunoassays for sulfathiazole.. https://doi.org/10.1007/s00216-004-2683-1

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