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Multiple sclerosis as a generalized CNS disease--comparative microarray analysis of normal appearing white matter and lesions in secondary progressive MS.

Raija L P Lindberg·Corline J A De Groot·Ulrich Certa·Rivka Ravid·Francine Hoffmann·Ludwig Kappos·David Leppert
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Abstract

We used microarrays to compare the gene expression profile in active lesions and donor-matched normal appearing white matter (NAWM) from brain autopsy samples of patients with secondary progressive multiple sclerosis (MS) with that from controls who died from non-neurological diseases. The 123 genes in lesions, and 47 genes in NAWM(MS) were differentially expressed. Lesions distinguished from NAWM(MS) by a higher expression of genes related to immunoglobulin synthesis and neuroglial differentiation, while cellular immune response elements were equally dysregulated in both tissue compartments. Current results provide molecular evidence of a continuum of dysfunctional homeostasis and inflammatory changes between lesions and NAWM(MS), and support the concept of MS pathogenesis being a generalised process that involves the entire CNS.

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BrainGene Expression ProfilingGene Expression RegulationHumansMultiple SclerosisOligonucleotide Array Sequence AnalysisReverse Transcriptase Polymerase Chain ReactionComparative StudyJournal ArticleResearch Support, Non-U.S. Gov't

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Raija L P Lindberg, Corline J A De Groot, Ulrich Certa, Rivka Ravid, Francine Hoffmann, Ludwig Kappos, David Leppert. 2004. Multiple sclerosis as a generalized CNS disease--comparative microarray analysis of normal appearing white matter and lesions in secondary progressive MS.. https://doi.org/10.1016/j.jneuroim.2004.03.011

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