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PubMed · 15228337

DNA displacement assay integrated into microfluidic channels.

Rebecca A Zangmeister·Michael J Tarlov
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Abstract

This paper describes the development of a unique fluorescence-based DNA diagnostic microfluidic assay that does not require labeling of the target sequence prior to analysis. The assay is based on the displacement of a short sacrificial fluorescent-tagged indicator oligomer by a longer untagged target sequence as it is electrophoresed through a DNA-containing hydrogel plug immobilized in a microfluidic channel. The distinct advantages of this assay are the short sensing times, as a result of directed electrophoretic transport of target DNA to the sensing element, combined with the ability to detect nonlabeled target DNA.

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DNAElectrophoresis, CapillaryFluorescent DyesHydrogel, Polyethylene Glycol DimethacrylateMicrofluidicsSensitivity and SpecificitySurface PropertiesJournal ArticleResearch Support, U.S. Gov't, Non-P.H.S.

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Rebecca A Zangmeister, Michael J Tarlov. 2004-07-01. DNA displacement assay integrated into microfluidic channels.. https://doi.org/10.1021/ac035238v

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