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Reliable high-throughput functional screening with 3-FABS.

Claudio Dalvit·Elena Ardini·Gian Paolo Fogliatto·Nicola Mongelli·Marina Veronesi
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Abstract

An NMR method called 3-FABS has extended the capabilities of NMR, enabling rapid, efficient and reliable high-throughput functional screening for the identification of inhibitors and for measuring their 50% mean inhibition concentration (IC(50)) with accuracy. The substrate is tagged with a CF(3) moiety and (19)F NMR spectroscopy is used for the detection of the substrate and product components. We provide comprehensive insight into 3-FABS, a discussion of its strength and weakness when compared with other HTS techniques and a presentation of some of its applications to the screening of different enzymes and to multiple screening.

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Drug Evaluation, PreclinicalFluorine RadioisotopesMagnetic Resonance SpectroscopySubstrate SpecificityTechnology, PharmaceuticalJournal ArticleReview

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BibTeXRIS

Claudio Dalvit, Elena Ardini, Gian Paolo Fogliatto, Nicola Mongelli, Marina Veronesi. 2004-07-15. Reliable high-throughput functional screening with 3-FABS.. https://doi.org/10.1016/s1359-6446(04)03161-7

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