Surface attachment of nanoparticles using oligonucleotides.

M Andersson; K Elihn; K Fromell; K D Caldwell

doi:10.1016/j.colsurfb.2003.12.007

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Surface attachment of nanoparticles using oligonucleotides.

M Andersson·K Elihn·K Fromell·K D Caldwell

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Abstract

Colloidal polymer particles are widely used in a variety of applications ranging from chromatography to surface modified bioreactors in protein arrays. In the present study, surface attachment of polystyrene particles to a polystyrene substrate has been performed using oligonucleotide hybridization. Thiolated complementary oligomers of cytosine and guanine have been covalently coupled to a pyridyl disulphide (PDS) modified polyethyleneglycol tether, forming part of a triblock copolymer which is adsorbed to the polystyrene surfaces via hydrophobic polypropylene oxide center blocks. The ability to withstand shear forces was studied using a laminar flow cell and the uptake of oligomers on the particles was quantified using two complementary techniques: UV-spectroscopy and sedimentation field flow fractionation. The possibility to tether particles in a flow cell suitable for practical use in e.g. a FIA-system is demonstrated.

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Binding Sites Disulfides Microscopy, Electron, Scanning Models, Chemical Nanotechnology Nucleic Acid Hybridization Oligonucleotides Polyethylene Glycols Polymers Polystyrenes Stress, Mechanical Surface Properties Ultraviolet Rays Journal Article Research Support, Non-U.S. Gov't

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M Andersson, K Elihn, K Fromell, K D Caldwell. 2004-04-01. Surface attachment of nanoparticles using oligonucleotides.. https://doi.org/10.1016/j.colsurfb.2003.12.007

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