Expression profiling by high-throughput immunohistochemistry.

Anthony Warford; William Howat; John McCafferty

doi:10.1016/j.jim.2004.04.010

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Expression profiling by high-throughput immunohistochemistry.

Anthony Warford·William Howat·John McCafferty

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Abstract

Immunohistochemistry (IHC) provides valuable information on expression of proteins within tissues at a cellular and subcellular level. Recent developments in the practice of IHC now make it possible to contemplate using this technique as a high-throughput expression profiling system. Advances have been made in creation and use of tissue microarrays, in automated IHC and in image capture/analysis. Each of these technologies are reviewed and issues surrounding their use considered. The success of high-throughput IHC is also dependent on both generation and screening of appropriate antibodies. Antibody-related issues which are likely to affect the success of high-throughput IHC, such as specificity, sensitivity, fixation choice, etc., are also considered.

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Animals Antibodies Antibody Specificity Flow Cytometry Gene Expression Profiling Immunohistochemistry Mice Journal Article Review

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Anthony Warford, William Howat, John McCafferty. 2004. Expression profiling by high-throughput immunohistochemistry.. https://doi.org/10.1016/j.jim.2004.04.010

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