Agrobacterium T-DNA integration: molecules and models.

Tzvi Tzfira; Jianxiong Li; Benoît Lacroix; Vitaly Citovsky

doi:10.1016/j.tig.2004.06.004

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Agrobacterium T-DNA integration: molecules and models.

Tzvi Tzfira·Jianxiong Li·Benoît Lacroix·Vitaly Citovsky

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Abstract

Genetic transformation mediated by Agrobacterium involves the transfer of a DNA molecule (T-DNA) from the bacterium to the eukaryotic host cell, and its integration into the host genome. Whereas extensive work has revealed the biological mechanisms governing the production, Agrobacterium-to-plant cell transport and nuclear import of the Agrobacterium T-DNA, the integration step remains largely unexplored, although several different T-DNA integration mechanisms have been suggested. Recent genetic and functional studies have revealed the importance of host proteins involved in DNA repair and maintenance for T-DNA integration. In this article, we review our understanding of the specific function of these proteins and propose a detailed model for integration.

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Active Transport, Cell Nucleus DNA Repair DNA Transposable Elements DNA, Bacterial Models, Genetic Recombination, Genetic Rhizobium Transformation, Genetic Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.Review

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Tzvi Tzfira, Jianxiong Li, Benoît Lacroix, Vitaly Citovsky. 2004. Agrobacterium T-DNA integration: molecules and models.. https://doi.org/10.1016/j.tig.2004.06.004

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