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PubMed · 15262448

High-throughput method for detecting DNA methylation.

Peng Hou·Meiju Ji·Song Li·Nongyue He·Zuhong Lu
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Abstract

Aberrant DNA methylation of CpG site is among the earliest and most frequent alterations in cancer. Detection of promoter hypermethylation of cancer-related gene may be useful for cancer diagnosis or the detection of recurrence. However, most of the studies have focused on a single gene only and gave little information about the concurrent methylation status of multiple genes. In this study, we attempted to develop a microarray method coupled with linker-PCR for detecting methylation status of multiple genes in the tumor tissue. A series of synthesized oligonucleotides were synthesised and purified to completely match with 16 investigated targets. Then they were immobilized on the aldehyde-coated glass slide to fabricate a DNA microarray for detecting methylation status of these genes. The results indicated that these genes were all methylated in the positive control. However, no methylated was found in these genes for the negative control. Only p16 and p15 genes were methylated in investigated genes for the gastric tumor tissue, whereas others were not methylated. The above results were validated by bisulfite DNA sequencing. Our experiments successfully demonstrated that the DNA microarray could be applied as a high-throughput tool to determine methylation status of the investigated genes.

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AutomationBase SequenceCell Cycle ProteinsCpG IslandsCyclin-Dependent Kinase Inhibitor p15DNADNA MethylationGenes, p16Genetic TechniquesGlassHumansMolecular Sequence DataNucleic Acid HybridizationOligonucleotide Array Sequence AnalysisOligonucleotidesPolymerase Chain ReactionStomach NeoplasmsSulfitesTime FactorsTumor Suppressor ProteinsJournal ArticleResearch Support, Non-U.S. Gov't

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Peng Hou, Meiju Ji, Song Li, Nongyue He, Zuhong Lu. 2004-08-31. High-throughput method for detecting DNA methylation.. https://doi.org/10.1016/j.jbbm.2004.05.001

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