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High-frequency ultrasound database profiling growth, development, and cardiovascular function in C57BL/6J mouse fetuses.

Christopher F Spurney·Linda Leatherbury·Cecilia W Lo
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Abstract

BACKGROUND: High-frequency ultrasound is effective for noninvasive phenotypic analysis of cardiovascular development and function in mutagenized fetal mice. However, lacking is a normative database of echocardiographic variables for monitoring growth and cardiovascular function. METHODS: C57BL/6J fetal mice were scanned in utero using an ultrasound system with a 15-MHz linear phased-array transducer. Pregnant mothers were anesthetized with 1% isoflurane mixed with 100% oxygen. Quantitative variables for monitoring fetal growth and cardiac function were obtained in several imaging planes and modalities. RESULTS: Fetal growth measurements increased linearly. Inflow velocities showed significant A-wave dominance. The E wave progressively increased during development. Cardiac function was best assessed through M-mode analysis, but short-axis images were difficult to obtain. Spectral Doppler was readily obtained and the myocardial performance index was calculated. CONCLUSIONS: These results provide an essential foundation for the evaluation of cardiovascular defects in mutagenized and transgenic mice.

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AnimalsEchocardiographyEmbryonic and Fetal DevelopmentFemaleFetal HeartHeart Defects, CongenitalMiceMice, Inbred C57BLPregnancyUltrasonography, PrenatalJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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Christopher F Spurney, Linda Leatherbury, Cecilia W Lo. 2004. High-frequency ultrasound database profiling growth, development, and cardiovascular function in C57BL/6J mouse fetuses.. https://doi.org/10.1016/j.echo.2004.04.028

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