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A single, multiplex analysis for all relevant activating NRAS gene mutations using heteroduplex generators.

Carolina Belli·Derrick J Bowen·Carlos De Brasi·Irene Larripa
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Abstract

We describe a multiplex polymerase chain reaction (PCR)-based test that detected all relevant NRAS activating mutations using a single PCR followed directly by electrophoresis. The test uses a Universal Heteroduplex Generator (UHG) to detect exon-2 (codon 61) NRAS mutations in multiplex with an UHG for exon-1 (codons 12 and 13). The method differentiated all 19 relevant mutations in these exons and showed a mutation independent sensitivity of approximately 6%. The sensitive, specific detection of all NRAS activating mutations using this single rapid test represents a minimum workload and could be applied readily for large-scale screening and for routine analysis.

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Base SequenceGenes, rasHeteroduplex AnalysisHumansMolecular Sequence DataMutationPolymerase Chain ReactionSensitivity and SpecificityEvaluation StudyJournal ArticleResearch Support, Non-U.S. Gov't

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Carolina Belli, Derrick J Bowen, Carlos De Brasi, Irene Larripa. 2004. A single, multiplex analysis for all relevant activating NRAS gene mutations using heteroduplex generators.. https://doi.org/10.1111/j.1365-2141.2004.05079.x

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