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Apoptotic/mytogenic pathways during human heart development.

Paolo Fiorina·Domenico Corradi·Silvana Pinelli·Roberta Maestri·Costanza Lagrasta·Mauro Buscaglia·Alberto Davalli·Franco Folli·Ettore Astorri
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Abstract

OBJECTIVE: The aim of our study was to assess myocytes apoptosis/mitosis and associated intracellular signalling pathways during heart development. SETTING AND PATIENTS: Eight human fetal hearts (at different gestation ages) and seven human adult hearts were chosen as controls (five normal and two pathological) and studied from both a histological and a molecular point of view. RESULTS: Our results are as follows: (i) all Shc isoforms are expressed and activated in the human fetal heart; (ii) a progressive fading of Shc and ERK expression are evident during gestation; (iii) JNK is present but it is not activated in the human fetal heart; (iv) CD95 is present in the first week of gestation and fades progressively; (v) apoptotic/proliferative processes are present in the early gestation phase and fades progressively; (vi) in the human heart, Shc isoform with medium weight is 55 kD and not 52 kD and it is upregulated in adult myocardial ischaemia. CONCLUSIONS: Myocyte underwent apoptosis/mitosis during gestation. Shc isoforms, together with ERK maintain the homeostasis of the heart.

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Adaptor Proteins, Signal TransducingAdultApoptosisCase-Control StudiesCell ProliferationFemaleFetal HeartGestational AgeHeartHumansJNK Mitogen-Activated Protein KinasesMAP Kinase Kinase 4MaleMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase KinasesMonocytesMyocardiumPhosphorylationShc Signaling Adaptor ProteinsSrc Homology 2 Domain-Containing, Transforming Protein 1fas ReceptorJournal ArticleResearch Support, Non-U.S. Gov't

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Paolo Fiorina, Domenico Corradi, Silvana Pinelli, Roberta Maestri, Costanza Lagrasta, Mauro Buscaglia, Alberto Davalli, Franco Folli, Ettore Astorri. 2004. Apoptotic/mytogenic pathways during human heart development.. https://doi.org/10.1016/j.ijcard.2003.08.010

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