[Low temperature effects on sarcoplasmic reticulum membrane permeability for Ca2+].

G F Zhegunov; A M Belous

PubMed · 153027

[Low temperature effects on sarcoplasmic reticulum membrane permeability for Ca2+].

Abstract

The effect of rapid freezing down to--196 degree C was studied as applied to the functional activity of sarcoplasmic reticulum (SR) membranes Ca2+-pump. It is found that the SR Ca-transport system is damaged under the effect of low temperatures: the transport activity of Ca ions across the reticulum membrane is reduced simultaneously with a slight increase in the ATPase activity. The rate of Ca ions accumulation by SR vesicles decreases and the magnitude of Ca: ATP ratio is reduced. The damage of the SR membrane Ca-transport system after freezing is related to an increase in the rate of the accumulated Ca2+ rapid leak from the vesicles through the system of active transport at the moment of ATPase "work" and to an increase in the rate of passive diffusion.

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Animals Biological Transport, Active Calcium Calcium-Transporting ATPases Freezing Intracellular Membranes Kinetics Permeability Rabbits Sarcoplasmic Reticulum Journal Article

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G F Zhegunov, A M Belous. [Low temperature effects on sarcoplasmic reticulum membrane permeability for Ca2+].. https://pubmed.ncbi.nlm.nih.gov/153027/

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