Altered CD45 isoform expression affects lymphocyte function in CD45 Tg mice.

Elma Z Tchilian; Ritu Dawes; Lisa Hyland; Maria Montoya; Agnes Le Bon; Persephone Borrow; Sam Hou; David Tough; Peter C L Beverley

doi:10.1093/intimm/dxh135

PubMed · 15302847

Altered CD45 isoform expression affects lymphocyte function in CD45 Tg mice.

Elma Z Tchilian·Ritu Dawes·Lisa Hyland·Maria Montoya·Agnes Le Bon·Persephone Borrow·Sam Hou·David Tough·Peter C L Beverley

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Abstract

Transgenic mice have been constructed expressing high (CD45RABC) and low (CD45R0) molecular weight CD45 isoforms on a CD45-/- background. Phenotypic analysis and in vivo challenge of these mice with influenza and lymphocytic choriomeningitis viruses shows that T cell differentiation and peripheral T cell function are related to the level of CD45 expression but not to which CD45 isoform is expressed. In contrast, B cell differentiation is not restored, irrespective of the level of expression of a single isoform. All CD45 trangenic mice have T cells with an activated phenotype and increased T cell turnover. These effects are more prominent in CD8 than CD4 cells. The transgenic mice share several properties with humans expressing variant CD45 alleles and provide a model to understand immune function in variant individuals.

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Animals B-Lymphocytes Immunophenotyping Leukocyte Common Antigens Lymphocytic choriomeningitis virus Mice Mice, Inbred C57BL Mice, Inbred DBA Mice, Transgenic Orthomyxoviridae Protein Isoforms T-Lymphocytes Journal Article

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Elma Z Tchilian, Ritu Dawes, Lisa Hyland, Maria Montoya, Agnes Le Bon, Persephone Borrow, Sam Hou, David Tough, Peter C L Beverley. 2004-08-09. Altered CD45 isoform expression affects lymphocyte function in CD45 Tg mice.. https://doi.org/10.1093/intimm%2Fdxh135

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