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DNA-functionalized nanotube membranes with single-base mismatch selectivity.

Punit Kohli·C Chad Harrell·Zehui Cao·Rahela Gasparac·Weihong Tan·Charles R Martin
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Abstract

We describe synthetic membranes in which the molecular recognition chemistry used to accomplish selective permeation is DNA hybridization. These membranes contain template-synthesized gold nanotubes with inside diameters of 12 nanometers, and a "transporter" DNA-hairpin molecule is attached to the inside walls of these nanotubes. These DNA-functionalized nanotube membranes selectively recognize and transport the DNA strand that is complementary to the transporter strand, relative to DNA strands that are not complementary to the transporter. Under optimal conditions, single-base mismatch transport selectivity can be obtained.

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Base Pair MismatchBiological TransportDNADeoxyribonucleases, Type II Site-SpecificGoldMembranes, ArtificialNanotubesNucleic Acid ConformationNucleic Acid HybridizationJournal ArticleResearch Support, U.S. Gov't, Non-P.H.S.

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Punit Kohli, C Chad Harrell, Zehui Cao, Rahela Gasparac, Weihong Tan, Charles R Martin. 2004-08-13. DNA-functionalized nanotube membranes with single-base mismatch selectivity.. https://doi.org/10.1126/science.1100024

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