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X-ray high-resolution vascular network imaging.

F Plouraboue·P Cloetens·C Fonta·A Steyer·F Lauwers·J P Marc-Vergnes
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Abstract

This paper presents the first application of high-resolution X-ray synchrotron tomography to the imaging of large microvascular networks in biological tissue samples. This technique offers the opportunity of analysing the full three-dimensional vascular network from the micrometre to the millimetre scale. This paper presents the specific sample preparation method and the X-ray imaging procedure. Either barium or iron was injected as contrast agent in the vascular network. The impact of the composition and concentration of the injected solution on the X-ray synchrotron tomography images has been studied. Two imaging modes, attenuation and phase contrast, are compared. Synchrotron high-resolution computed tomography offers new prospects in the three-dimensional imaging of in situ biological vascular networks.

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AnimalsBlood VesselsCapillariesCerebrovascular CirculationHumansImage Processing, Computer-AssistedMicrocirculationRatsSensitivity and SpecificitySynchrotronsTomography, X-RayJournal Article

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F Plouraboue, P Cloetens, C Fonta, A Steyer, F Lauwers, J P Marc-Vergnes. 2004. X-ray high-resolution vascular network imaging.. https://doi.org/10.1111/j.0022-2720.2004.01362.x

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