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PubMed · 15315770

Rapid, automated screening method for enzymatic transformations using a robotic system and supercritical fluid chromatography.

Li Di·Oliver J McConnell·Edward H Kerns·Alan G Sutherland
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Abstract

An automated screening method was developed for enzymatic transformations using a robotic system and rapid chiral supercritical fluid chromatography (SFC) analysis with a run time of 1.5 min. The method accelerates the enzyme selection process for screening biocatalysts, where a large number of enzymes are evaluated for activity and enantioselectivity. Kinetic resolution of secondary alcohols by enzymatic transesterification was used as a prototype for method development. The rapid automated method can be used effectively for screening enzymes and optimizing reaction conditions in biocatalysis.

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AutomationBiotransformationChromatography, Supercritical FluidEnzymesRoboticsStereoisomerismJournal Article

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Li Di, Oliver J McConnell, Edward H Kerns, Alan G Sutherland. 2004-10-05. Rapid, automated screening method for enzymatic transformations using a robotic system and supercritical fluid chromatography.. https://doi.org/10.1016/j.jchromb.2004.06.033

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