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PubMed · 15324554

SARS coronavirus detection.

Andreas Nitsche·Brunhilde Schweiger·Heinz Ellerbrok·Matthias Niedrig·Georg Pauli
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Abstract

We developed a set of three real-time reverse transcription-polymerase chain reaction (PCR) assays that amplify three different regions of the SARS-associated coronavirus (SARS-CoV), can be run in parallel or in a single tube, and can detect <10 genome equivalents of SARS-CoV. The assays consider all currently available SARS-CoV sequences and are optimized for two prominent real-time PCR platforms.

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HumansRNA, ViralReverse Transcriptase Polymerase Chain ReactionSevere acute respiratory syndrome-related coronavirusSensitivity and SpecificitySevere Acute Respiratory SyndromeComparative StudyEvaluation StudyJournal Article

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Andreas Nitsche, Brunhilde Schweiger, Heinz Ellerbrok, Matthias Niedrig, Georg Pauli. 2004. SARS coronavirus detection.. https://doi.org/10.3201/eid1007.030678

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