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Rapid identification of chromosomes carrying silver-stained nucleolus-organizing regions: application to case of 21/21 Robertsonian translocation.

C H Buys·J Osinga·W L Gouw·G J Anders
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Abstract

The use of a combination of transmitted light and epiluminescence after silver and fluorescent staining of chromosome preparations makes it possible to achieve simultaneous visualization of silver-stained NORs and flourescent chromosomes. This technique permits exact localization of silver precipitates on normal and BrdU-substituted chromosomes. After previous silver impregnation, fluorescent staining by actinomycin-daunomycin-DAPI was used to induce a banding pattern that enables identification of specific chromosomes while observing silver-stained NORs at the same time. Application of this method to a Down's syndrome patient revealed a 21/21 Robertsonian translocation with NOR'S eliminated.

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Cell NucleolusCells, CulturedChromosome BandingChromosomes, HumanChromosomes, Human, 21-22 and YDown SyndromeHumansLymphocytesSilverTranslocation, GeneticJournal Article

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C H Buys, J Osinga, W L Gouw, G J Anders. 1978-10-31. Rapid identification of chromosomes carrying silver-stained nucleolus-organizing regions: application to case of 21/21 Robertsonian translocation.. https://doi.org/10.1007/bf00295410

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