Nucleotide-dependent interactions between a fork junction-RNA polymerase complex and an AAA+ transcriptional activator protein.

W V Cannon; J Schumacher; M Buck

doi:10.1093/nar/gkh755

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Nucleotide-dependent interactions between a fork junction-RNA polymerase complex and an AAA+ transcriptional activator protein.

W V Cannon·J Schumacher·M Buck

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Abstract

Enhancer-dependent transcriptional activators that act upon the sigma54 bacterial RNA polymerase holoenzyme belong to the extensive AAA+ superfamily of mechanochemical ATPases. Formation and collapse of the transition state for ATP hydrolysis engenders direct interactions between AAA+ activators and the sigma54 factor, required for RNA polymerase isomerization. A DNA fork junction structure present within closed complexes serves as a nucleation point for the DNA melting seen in open promoter complexes and restricts spontaneous activator-independent RNA polymerase isomerization. We now provide physical evidence showing that the ADP.AlF(x) bound form of the AAA+ domain of the transcriptional activator protein PspF changes interactions between sigma54-RNA polymerase and a DNA fork junction structure present in the closed promoter complex. The results suggest that one functional state of the nucleotide-bound activator serves to alter DNA binding by sigma54 and sigma54-RNA polymerase and appears to drive events that precede DNA opening. Clear evidence for a DNA-interacting activity in the AAA+ domain of PspF was obtained, suggesting that PspF may make a direct contact to the DNA component of a basal promoter complex to promote changes in sigma54-RNA polymerase-DNA interactions that favour open complex formation. We also provide evidence for two distinct closed promoter complexes with differing stabilities.

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Adenosine Diphosphate Adenosine Triphosphatases Aluminum Compounds Binding Sites DNA Footprinting DNA, Bacterial DNA-Binding Proteins DNA-Directed RNA Polymerases Escherichia coli Proteins Fluorides Macromolecular Substances Promoter Regions, Genetic Protein Structure, Tertiary RNA Polymerase Sigma 54 Sigma Factor Trans-Activators Journal Article Research Support, Non-U.S. Gov't

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W V Cannon, J Schumacher, M Buck. 2004-08-27. Nucleotide-dependent interactions between a fork junction-RNA polymerase complex and an AAA+ transcriptional activator protein.. https://doi.org/10.1093/nar%2Fgkh755

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