Solid-phase synthesis of circular oligonucleotides.

Enrique Pedroso; Nuria Escaja; Miriam Frieden; Anna Grandas

doi:10.1385/1-59259-823-4:101

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Solid-phase synthesis of circular oligonucleotides.

Enrique Pedroso·Nuria Escaja·Miriam Frieden·Anna Grandas

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Abstract

A protocol for the straightforward preparation of small circular oligodeoxyribonucleotides (2-28 nt) is reported. The assembly of the oligonucleotide chain (standard phosphoramidite chemistry) and cyclization by the phosphotriester method take place on a tailor-made nucleotide-derivatized solid support. Although cyclization yields are moderate, the procedure exploits a synthesis design that allows selective cleavage of the circular oligonucleotide from the support, which facilitates isolation of the target molecule by simple filtration.

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Chromatography, High Pressure Liquid Electrophoresis, Polyacrylamide Gel Oligonucleotides Journal Article Research Support, U.S. Gov't, P.H.S.

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Enrique Pedroso, Nuria Escaja, Miriam Frieden, Anna Grandas. 2005. Solid-phase synthesis of circular oligonucleotides.. https://doi.org/10.1385/1-59259-823-4%3A101

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