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PubMed · 15333903

Base-modified oligonucleotides with increased duplex stability: pyrazolo[3,4-d] pyrimidines replacing purines.

Frank Seela·Yang He·Junlin He·Georg Becher·Rita Kröschel·Matthias Zulauf·Peter Leonard
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Abstract

Oligonucleotides incorporating 8-aza-7-dazapurines (pyrazolo[3,4-d]pyrimidines) were synthesized. The corresponding nucleosides were prepared and were converted into phosphoramidites. The oligonucleotide duplex stability was studied and was compared to that of the parent compounds containing the canonical purine nucleosides. The presence of 7-halogeno or 7-alkynyl substituents increases the duplex stability significantly.

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Base SequenceChromatography, Thin LayerNuclear Magnetic Resonance, BiomolecularOligonucleotidesPurinesPyrazolesPyrimidinesThermodynamicsJournal ArticleResearch Support, Non-U.S. Gov't

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Frank Seela, Yang He, Junlin He, Georg Becher, Rita Kröschel, Matthias Zulauf, Peter Leonard. 2005. Base-modified oligonucleotides with increased duplex stability: pyrazolo[3,4-d] pyrimidines replacing purines.. https://doi.org/10.1385/1-59259-823-4%3A165

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