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Nucleic acid library construction using synthetic DNA constructs.

Hani S Zaher·Peter J Unrau
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Abstract

This chapter outlines seven synthetic and molecular biology techniques that allow the controlled synthesis of nucleic acid libraries. Specifically: (1) The high-diversity chemical synthesis of point mutations; (2) the high-diversity chemical synthesis of point deletions; (3) the split-bead approach for constructing point mutation or deletion libraries with limited sequence diversity; (4) pool deprotection, gel purification, and quality-control techniques; (5) large-scale polymerase chain reaction amplification for the generation of high-diversity double-stranded deoxyribonucleic acid libraries; (6) type II restriction enzyme digestion techniques for the construction of long-sequence libraries containing minimal fixed sequence; and (7) extension techniques for the rapid synthesis of long, low-diversity oligonucleotide sequences.

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DNANucleic AcidsPoint MutationPolymerase Chain ReactionJournal ArticleResearch Support, Non-U.S. Gov't

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Hani S Zaher, Peter J Unrau. 2005. Nucleic acid library construction using synthetic DNA constructs.. https://doi.org/10.1385/1-59259-823-4%3A359

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