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PubMed · 15333933

Cloning, expression, purification, crystallization and initial crystallographic analysis of transcription factor DksA from Escherichia coli.

Marina N Vassylyeva·Anna A Perederina·Vladimir Svetlov·Shigeyuki Yokoyama·Irina Artsimovitch·Dmitry G Vassylyev
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Abstract

The Escherichia coli gene encoding a regulator of stringent response and virulence, DksA, which contains a canonical Zn finger motif, was cloned and expressed, and the purified protein was crystallized by the hanging-drop vapor-diffusion technique in two different space groups, P2(1)2(1)2(1) (a = 91.32, b = 96.59, c = 117.48 A) and C222 (a = 80.6, b = 115.1, c = 149.57 A). The crystals belonging to space group P2(1)2(1)2(1), improved by macroseeding, diffract beyond 2.0 A at a synchrotron. Three complete atomic resolution multiple anomalous dispersion diffraction data sets were collected from the same crystal of the P2(1)2(1)2(1) crystal form at the absorption edge for Zn atoms.

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Cloning, MolecularCrystallizationCrystallography, X-RayEscherichia coliEscherichia coli ProteinsModels, MolecularReverse Transcriptase Polymerase Chain ReactionSynchrotronsZinc FingersJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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Marina N Vassylyeva, Anna A Perederina, Vladimir Svetlov, Shigeyuki Yokoyama, Irina Artsimovitch, Dmitry G Vassylyev. 2004-08-26. Cloning, expression, purification, crystallization and initial crystallographic analysis of transcription factor DksA from Escherichia coli.. https://doi.org/10.1107/s0907444904015033

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