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Using analytical multidimensional isocratic HPLC methods of separation to isolate active constituents in natural products.

Victor Wong·Alan P Sweeney·R Andrew Shalliker
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Abstract

Multidimensional reversed-phase HPLC was employed to isolate the active constituents from a crude extract of Clerodendrum floribundum R. Br. These active constituents were known to have bioactivity against the enzyme xanthine oxidase and potentially could be employed in the treatment of gout. Using a multidimensional separation approach, rapid isolation of the active constituents was achieved from a complex sample matrix. As each separation dimension was isocratic, no equilibration time was required and consequently the technique shows promise in the scale up to preparative levels where high throughput is important.

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Biological ProductsChromatography, High Pressure LiquidClerodendrumEnzyme InhibitorsGoutHumansPlant ExtractsXanthine OxidaseJournal ArticleResearch Support, Non-U.S. Gov't

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Victor Wong, Alan P Sweeney, R Andrew Shalliker. 2004. Using analytical multidimensional isocratic HPLC methods of separation to isolate active constituents in natural products.. https://doi.org/10.1002/jssc.200301589

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