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Improving identification of differentially expressed genes in microarray studies using information from public databases.

Richard D Kim·Peter J Park
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Abstract

We demonstrate that the process of identifying differentially expressed genes in microarray studies with small sample sizes can be substantially improved by extracting information from a large number of datasets accumulated in public databases. The improvement comes from more reliable estimates of gene-specific variances based on other datasets. For a two-group comparison with two arrays in each group, for example, the result of our method was comparable to that of a t-test analysis with five samples in each group or to that of a regularized t-test analysis with three samples in each group. Our results are further improved by weighting the results of our approach with the regularized t-test results in a hybrid method.

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Databases, GeneticGene Expression ProfilingGene Expression RegulationOligonucleotide Array Sequence AnalysisComparative StudyJournal Article

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Richard D Kim, Peter J Park. 2004-08-26. Improving identification of differentially expressed genes in microarray studies using information from public databases.. https://doi.org/10.1186/gb-2004-5-9-r70

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