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PubMed · 15354560

Characterization of polyacrylamide based monolithic columns.

Fatima M Plieva·Jonatan Andersson·Igor Yu Galaev·Bo Mattiasson
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Abstract

Supermacroporous monolithic polyacrylamide (pAAm)-based columns have been prepared by radical cryo-copolymerization (copolymerization in the moderately frozen system) of acrylamide with functional co-monomer, allyl glycidyl ether (AGE), and cross-linker N,N'-methylene-bis-acrylamide (MBAAm) directly in glass columns (ID 10 mm). The monolithic columns have uniform supermacroporous sponge-like structure with interconnected supermacropores of pore size 5-100 microm. The monoliths can be dried and stored in the dry state. High mechanical stability of the monoliths allowed sterilization by autoclaving. Column-to-column reproducibility of pAAm-monoliths was demonstrated on 5 monolithic columns from different batches prepared under the same cryostructuration conditions.

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AcetoneAcrylamidesAcrylic ResinsBlood ProteinsChromatographyCopperCryogelsEpoxy CompoundsFibronectinsFreezingHydrogelsIonsMicroscopy, Electron, ScanningPolymersPressureTemperatureTime FactorsPolyacrylamidesJournal ArticleResearch Support, Non-U.S. Gov't

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Fatima M Plieva, Jonatan Andersson, Igor Yu Galaev, Bo Mattiasson. 2004. Characterization of polyacrylamide based monolithic columns.. https://doi.org/10.1002/jssc.200401836

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