Backbone thioester exchange: a new approach to evaluating higher order structural stability in polypeptides.

Matthew G Woll; Samuel H Gellman

doi:10.1021/ja046891i

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Backbone thioester exchange: a new approach to evaluating higher order structural stability in polypeptides.

Matthew G Woll·Samuel H Gellman

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Abstract

An amide bond has been replaced by a thioester in bovine pancreatic polypeptide (bPP) to allow rapid and reversible (dynamic) exchange of the alpha-helical segment with other thiols in solution. We have begun to study the higher order structural stability of bPP by measuring the equilibrium constant of the "backbone thioester exchange" (BTE) reaction. The extent to which the equilibrium (KBTE) favors one set of peptides over the other, which can be easily measured, can be directly correlated to the energy gained from favorable noncovalent interactions that occur between peptide segments on either side of the thioester bond (Kfold).

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Amino Acid Sequence Animals Cattle Models, Molecular Molecular Sequence Data Nuclear Magnetic Resonance, Biomolecular Pancreatic Polypeptide Protein Structure, Tertiary Structure-Activity Relationship Thermodynamics Tyrosine Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

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Matthew G Woll, Samuel H Gellman. 2004-09-15. Backbone thioester exchange: a new approach to evaluating higher order structural stability in polypeptides.. https://doi.org/10.1021/ja046891i

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