Yeast cytochrome c is a sequence-specific DNA-binding protein.

Anshu Bhatnagar; Pralhada Rao Raghavendra; Balla V Kranthi; Pundi N Rangarajan

doi:10.1016/j.bbrc.2004.07.044

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Yeast cytochrome c is a sequence-specific DNA-binding protein.

Anshu Bhatnagar·Pralhada Rao Raghavendra·Balla V Kranthi·Pundi N Rangarajan

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Abstract

A protein binding to the alcohol oxidase 2 upstream activation sequence (AOX2UAS) of the methylotropic yeast, Pichia pastoris, has been purified and identified as cytochrome c (cyt c). Cyt c purified from P. pastoris or Saccharomyces cerevisiae binds to AOX2UAS. Specific point mutations in AOX2UAS abolish cyt c binding. We conclude that yeast cyt c is a sequence-specific DNA-binding protein and may have a regulatory role in the nucleus.

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Amino Acid Sequence Base Sequence Binding Sites Cytochromes c DNA, Fungal DNA-Binding Proteins Fungal Proteins Molecular Sequence Data Pichia Point Mutation Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Sequence Homology, Amino Acid Species Specificity Comparative Study Journal Article

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Anshu Bhatnagar, Pralhada Rao Raghavendra, Balla V Kranthi, Pundi N Rangarajan. 2004-09-03. Yeast cytochrome c is a sequence-specific DNA-binding protein.. https://doi.org/10.1016/j.bbrc.2004.07.044

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