DNA transfection screening from single beads.

Boon-Ek Yingyongnarongkul; Mark Howarth; Tim Elliott; Mark Bradley

doi:10.1021/cc049945t

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PubMed · 15360210

DNA transfection screening from single beads.

Boon-Ek Yingyongnarongkul·Mark Howarth·Tim Elliott·Mark Bradley

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Abstract

The solid-phase synthesis of a library of arginine-containing lipid transfection agents on high-loading beads is described. The transfection activity of the cationic lipids was determined using compound cleaved from single beads (single-bead screening) and showed, in some cases, comparable or higher DNA transfection activities as compared to commercially available reagents. Lipids with one arginine headgroup and a cholesterol tail were found to be the most active, even though their DNA binding strength (retardation assays) was relatively weak. Single-bead screening of transfection activity facilitates the rapid analysis of libraries of transfection reagents and will allow the rapid optimization of gene delivery into cells, both in culture and in vivo.

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Arginine Cations Cell Line Cell Survival Combinatorial Chemistry Techniques Guanidine Humans Lipids Microspheres Transfection Journal Article Research Support, Non-U.S. Gov't

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Boon-Ek Yingyongnarongkul, Mark Howarth, Tim Elliott, Mark Bradley. DNA transfection screening from single beads.. https://doi.org/10.1021/cc049945t

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