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Defined structural changes occur in a nucleosome upon Amt1 transcription factor binding.

Cindy L White·Karolin Luger
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Abstract

Here, we study the binding of the transcription factor Amt1 to its recognition site near the dyad of a highly positioned nucleosome. We find that the DNA binding domain of Amt1 binds to nucleosomes with only threefold reduced affinity compared to free DNA. We show by fluorescence resonance energy transfer that factor binding at the nucleosomal dyad is accompanied by the partial dissociation of the DNA ends from the histone octamer surface; however, no dissociation or subtle rearrangements of histone subunits is observed. A poly(dA.dT) DNA sequence element adjacent to the transcription factor binding site appears to facilitate factor binding, but is not essential. The methods that we describe here characterize for the first time the subtle structural changes that occur upon transcription factor binding to nucleosomes, and demonstrate the ability of the nucleosome to structurally adapt in response to outside influences.

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AnimalsBase SequenceBinding SitesDNADNA FootprintingDNA-Binding ProteinsDeoxyribonuclease IFluorescence Resonance Energy TransferFungal ProteinsHistonesMolecular Sequence DataNucleosomesPoly dA-dTPromoter Regions, GeneticProtein BindingProtein ConformationTranscription FactorsXenopus laevisJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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Cindy L White, Karolin Luger. 2004-10-01. Defined structural changes occur in a nucleosome upon Amt1 transcription factor binding.. https://doi.org/10.1016/j.jmb.2004.07.080

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