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Time-resolved total internal reflection fluorescence study on hybridization of complementary single-stranded DNAs at a water/oil interface.

Shoji Ishizaka·Yuichi Ueda·Noboru Kitamura
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Abstract

Hybridization of complementary single-stranded DNAs (ssDNA) at a water/CCl4 interface was studied on the basis of picosecond total internal reflection fluorescence spectroscopy. Complementary ssDNAs dissolved in water were shown to produce the relevant double-stranded DNA (dsDNA) at a water/CCl4 interface in the presence of octadecylamine (ODA) in the oil phase, while hybridization between ssDNAs did not proceed in the water phase, as demonstrated by the fluorescence dynamics of ethidium bromide as a probe for the DNA structure. The structures of dsDNA and the roles of ODA in hybridization of ssDNA at the interface were discussed.

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AminesCarbon TetrachlorideDNA, Single-StrandedNucleic Acid ConformationNucleic Acid HybridizationOilsSolutionsSpectrometry, FluorescenceWaterJournal ArticleResearch Support, Non-U.S. Gov't

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Shoji Ishizaka, Yuichi Ueda, Noboru Kitamura. 2004-09-01. Time-resolved total internal reflection fluorescence study on hybridization of complementary single-stranded DNAs at a water/oil interface.. https://doi.org/10.1021/ac049612i

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