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Expression and bioactivity analysis of recombinant beta-CPP dimer.

Y J Jiang·Q Z Li·H B Yan·L J Geng
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Abstract

Beta-casein phosphopeptide (beta-CPP) is a bioactive peptide that carries different minerals, especially calcium. To investigate more effects of beta-CPP, eukaryotic expression vector of beta-CPP dimer was constructed and transfected into Chinese hamster ovary (CHO) cells. After selection, the cell lines stably expressing beta-CPP dimer were obtained, and the recombinant product was identified and purified. Activity assay of recombinant protein indicated that the recombinant beta-CPP dimer could improve Ca(2+) uptake of sperm, stimulate the proliferation of spleen cells, and induce apoptosis of some malignant tumor cells.

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AnimalsApoptosisCHO CellsCalciumCaseinsCell DivisionCricetinaeDimerizationGene ExpressionGenetic VectorsHT29 CellsHumansMaleMiceMice, Inbred BALB CNeoplasmsPC12 CellsPhosphopeptidesRabbitsRatsRecombinant ProteinsSpermatozoaSpleenTransfectionJournal ArticleResearch Support, Non-U.S. Gov't

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Y J Jiang, Q Z Li, H B Yan, L J Geng. 2004. Expression and bioactivity analysis of recombinant beta-CPP dimer.. https://doi.org/10.3168/jds.s0022-0302(04)73455-4

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