High-throughput microarray detection of olfactory receptor gene expression in the mouse.

Xinmin Zhang; Matthew Rogers; Huikai Tian; Xiaohong Zhang; Dong-Jing Zou; Jian Liu; Minghong Ma; Gordon M Shepherd; Stuart J Firestein

doi:10.1073/pnas.0405350101

PubMed · 15377787

High-throughput microarray detection of olfactory receptor gene expression in the mouse.

Xinmin Zhang·Matthew Rogers·Huikai Tian·Xiaohong Zhang·Dong-Jing Zou·Jian Liu·Minghong Ma·Gordon M Shepherd·Stuart J Firestein

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Abstract

The large number of olfactory receptor genes necessitates high throughput methods to analyze their expression patterns. We have therefore designed a high-density oligonucleotide array containing all known mouse olfactory receptor (OR) and V1R vomeronasal receptor genes. This custom array detected a large number of receptor genes, demonstrating specific expression in the olfactory sensory epithelium for approximately 800 OR genes previously designated as ORs based solely on genomic sequences. The array also enabled us to monitor the spatial and temporal distribution of gene expression for the entire OR family. Interestingly, OR genes showing spatially segregated expression patterns were also segregated on the chromosomes. This correlation between genomic location and spatial expression provides unique insights about the regulation of this large family of genes.

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Age Factors Animals Chromosomes Cluster Analysis Gene Expression Profiling Genome Lung Male Mice Mice, Inbred Strains Olfactory Mucosa Oligonucleotide Array Sequence Analysis Receptors, Odorant Testis Vomeronasal Organ Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

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Xinmin Zhang, Matthew Rogers, Huikai Tian, Xiaohong Zhang, Dong-Jing Zou, Jian Liu, Minghong Ma, Gordon M Shepherd, Stuart J Firestein. 2004-09-17. High-throughput microarray detection of olfactory receptor gene expression in the mouse.. https://doi.org/10.1073/pnas.0405350101

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