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PubMed · 15379303

[Interaction between osteoblasts and fibroblasts derived from same individual].

Ping Gong·Zhen Tan·Qing Zhao
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Abstract

OBJECTIVE: In order to determine the effect of factors pertaining to the development and metabolism of periodontal ligament, the author investigated the interaction between osteoblasts and fibroblasts. METHODS: The cell co-culture model was established by cell culture inserts. The expression difference of protein related to mineralization (ALP, Type I collagen) between these two kinds of cells cultured individually and co-cultured were evaluated. RESULTS: Co-culture changed the ALPase activity of the two kinds of cells greatly .The ALP expression of osteoblasts decreased, but the ALP expression of periodontal ligament fibroblasts increased. The Col-I expression of these two kinds of cells did not change significantly when they were co-cultured. CONCLUSION: Periodontal ligament fibroblast could restrain the osteogenesis function of osteoblast, while osteoblast could induce the differentiation of fibroblast when both of them existed in periodontal tissue.

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Alkaline PhosphataseCell Culture TechniquesCell DifferentiationCoculture TechniquesCollagen Type IFibroblastsHumansOsteoblastsOsteogenesisPeriodontal LigamentEnglish AbstractJournal ArticleResearch Support, Non-U.S. Gov't

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Ping Gong, Zhen Tan, Qing Zhao. 2004. [Interaction between osteoblasts and fibroblasts derived from same individual].. https://pubmed.ncbi.nlm.nih.gov/15379303/

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