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PubMed · 15382745

P450scc mutant nanostructuring for optimal assembly.

Paola Ghisellini·Cristina Paternolli·Mirco Antonini·Claudio Nicolini
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Abstract

Molecular modeling and protein engineering were synergically employed to improve the fabrication of cytochrome P450scc mutant nanostructures for biodevice assembly. The optimization of protein three-dimensional structure by molecular modeling was performed using two models: in vacuum and simulating the presence of a polar solvent. Calculations were performed on a model to predict a P450scc mutant which could improve the process of molecules' immobilization onto solid supports. Engineerized cytochrome P450scc thin films were prepared and characterized by various biophysical techniques such as pi-A isotherms, surface potential measurements, Brewster angle microscopy, UV-vis spectroscopy, circular dichroism, nanogravimetry, and electrochemical analysis. This paper takes into consideration biomolecules modified by protein engineering that represent a new and powerful approach for obtaining synthetic simpler artificial structures with new or improved properties (i.e., specificity, stability, sensitivity, etc.) useful for biosensors development.

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Amino Acid SequenceAmino Acid SubstitutionCloning, MolecularComputer SimulationCytochrome P-450 Enzyme SystemElectrochemistryEnzyme StabilityEscherichia coliGenetic EnhancementModels, MolecularMolecular Sequence DataMutagenesis, Site-DirectedNanotechnologyProtein ConformationProtein EngineeringSequence Analysis, ProteinStructure-Activity RelationshipTemperatureJournal ArticleResearch Support, Non-U.S. Gov't

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Paola Ghisellini, Cristina Paternolli, Mirco Antonini, Claudio Nicolini. 2004. P450scc mutant nanostructuring for optimal assembly.. https://doi.org/10.1109/tnb.2004.828267

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