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Multifunctional yeast high-copy-number shuttle vectors.

T W Christianson·R S Sikorski·M Dante·J H Shero·P Hieter
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Abstract

A set of four yeast shuttle vectors that incorporate sequences from the Saccharomyces cerevisiae 2 mu endogenous plasmid has been constructed. These yeast episomal plasmid (YEp)-type vectors (pRS420 series) differ only in their yeast selectable markers, HIS3, TRP1, LEU2 or URA3. The pRS420 plasmids are based on the backbone of a multifunctional phagemid, pBluescript II SK+, and share its useful properties for growth in Escherichia coli and manipulation in vitro. The pRS420 plasmids have a copy number of about 20 per cell, equivalent to that of YEp24. During non-selective yeast growth, pRS420 plasmids are lost through mitotic segregation at rates similar to other YEp vectors and yeast centromeric plasmid (YCp) vectors, in the range of 1.5-5% of progeny per doubling. The pRS420 series provides high-copy-number counterparts to the current pRS vectors [Sikorski and Hieter, Genetics 122 (1989) 19-27].

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Cloning, MolecularDNA ReplicationDNA, FungalGene AmplificationGenetic VectorsPlasmidsRepliconSaccharomyces cerevisiaeJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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T W Christianson, R S Sikorski, M Dante, J H Shero, P Hieter. 1992-01-02. Multifunctional yeast high-copy-number shuttle vectors.. https://doi.org/10.1016/0378-1119(92)90454-w

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