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PubMed · 15452336

SpeedScreen: The "missing link" between genomics and lead discovery.

Hartmut Zehender·Francois Le Goff·Natalie Lehmann·Ireos Filipuzzi·Lorenz M Mayr
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Abstract

SpeedScreen is a novel, label-free, in-solution, affinity-based selection methodology for high-throughput screening (HTS) developed at Novartis Pharma. The SpeedScreen protocol comprises in-solution affinity selection, followed by size exclusion chromatography in combination with microbore-liquid-chromatography/electrospray-ionization mass spectrometry (micro-LC/ESI-MS). The authors describe the basic concept behind assay development, HTS, and data analysis with the SpeedScreen technology. Advantages and limitations of SpeedScreen compared to alternative screening technologies are discussed, and an example is given from a SpeedScreen campaign applying this innovative affinity selection concept in HTS.

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Chromatography, GelChromatography, High Pressure LiquidDrug DesignEquipment DesignGenomicsLeadMass SpectrometryModels, TheoreticalProtein BindingSensitivity and SpecificitySpectrometry, Mass, Electrospray IonizationTechnology, PharmaceuticalJournal Article

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Hartmut Zehender, Francois Le Goff, Natalie Lehmann, Ireos Filipuzzi, Lorenz M Mayr. 2004. SpeedScreen: The "missing link" between genomics and lead discovery.. https://doi.org/10.1177/1087057104267605

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