Diffusion systems for evaluation of biomineralization.

L Silverman; A L Boskey

doi:10.1007/s00223-004-0019-y

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Diffusion systems for evaluation of biomineralization.

L Silverman·A L Boskey

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Abstract

A variety of in vitro study methods have been used to elucidate the roles of matrix molecules in biomineralization processes. Among these, gel diffusion-precipitation studies have proved to be an effective tool. This methodology is uniquely capable of characterizing the effects of matrix molecules on mineralization while only using very small quantities of material. Furthermore, gel methods have been extended for use as a mineralization assay system to characterize modified matrix molecules and synthetic analogues. Here we discuss the advantages and limitations of gelatin, agar, agarose, and other systems for studying the mechanisms of biomineralization.

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Agar Calcification, Physiologic Calcium Cell-Free System Chemical Precipitation Diffusion Durapatite Extracellular Matrix Gelatin Gels Phosphates Sepharose Evaluation Study Journal Article Research Support, U.S. Gov't, P.H.S.

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L Silverman, A L Boskey. 2004-10-07. Diffusion systems for evaluation of biomineralization.. https://doi.org/10.1007/s00223-004-0019-y

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