Polygalacturonic acid/endo-polygalacturonase system: a kinetic study in batch reactors.

Alberto Gallifuoco; Maria Cantarella; Paolo Viparelli; Mariagrazia Marucci

doi:10.1021/bp049853b

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Polygalacturonic acid/endo-polygalacturonase system: a kinetic study in batch reactors.

Alberto Gallifuoco·Maria Cantarella·Paolo Viparelli·Mariagrazia Marucci

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Abstract

The enzymatic depolymerization of the pectic substance polygalacturonic acid (PGA) is studied in batch reactor. The number-average molecular weight of native substrate is estimated, using a simple and quick technique, to be approximately 11.1 kDa, the polymeric chains consisting on average of 63 galacturonic acid units. The effect of enzyme concentration was studied varying biocatalyst loading from 6 to 242 mg/L. The experiments were repeated at substrate concentrations ranging from 0.5 to 5 g/L. Data obtained at both short reaction time (20 min) and prolonged enzyme action (up to 350 min) are correlated using different kinetic equations, and the parameter values are discussed.

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Bioreactors Computer Simulation Enzyme Activation Enzyme Stability Kinetics Models, Chemical Molecular Weight Oxidation-Reduction Pectins Polygalacturonase Protein Binding Substrate Specificity Comparative Study Evaluation Study Journal Article Research Support, Non-U.S. Gov't

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Alberto Gallifuoco, Maria Cantarella, Paolo Viparelli, Mariagrazia Marucci. Polygalacturonic acid/endo-polygalacturonase system: a kinetic study in batch reactors.. https://doi.org/10.1021/bp049853b

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